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Literature summary extracted from

  • Vottariello, F.; Costanzo, C.; Gotte, G.; Libonati, M.
    Zero-length dimers of ribonuclease A: further characterization and no evidence of cytotoxicity (2010), Bioconjug. Chem., 21, 635-645.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 E9A site-directed mutagenesis Bos taurus
4.6.1.18 K66A site-directed mutagenesis, no intramolecular bonds form in the K66A variant Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18 purification of the covalent RNase A oligomers by two-step cation exchange chromatography and two gel filtration steps Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 commercial preparation
-
 Bos taurus
-
4.6.1.18 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.18 additional information
-
 enzymatic activities of the various RNase A monomeric and zero-length or domain-swapped oligomeric species on single-stranded yeast or double-stranded poly(A)-poly(U) RNA, overview. The dimers show higher activity compared to the monomners Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 poly(A)-poly(U) + H2O the enzyme dimers degrade poly(A)-poly(U) dsRNA with an activity that increases with the increase of the oligomer's basicity Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.18 dimer besides dimers, also trimers and higher oligomers can be identified among the products of the covalently linking reaction, and the zero-length dimers appear not to be a unique species, but heterogeneous, overview. Quantification of the zero-length oligomers Bos taurus

Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 ribonuclease A
-
 Bos taurus
4.6.1.18 RNase A
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.18 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.18 5
-
 assay at Bos taurus